Recombinant translation initiation factor-1 of Wolbachia is an immunogenic excretory secretory protein that elicits Th2 mediated immune protection against Brugia malayi.
Identifieur interne : 003227 ( Main/Exploration ); précédent : 003226; suivant : 003228Recombinant translation initiation factor-1 of Wolbachia is an immunogenic excretory secretory protein that elicits Th2 mediated immune protection against Brugia malayi.
Auteurs : Jeetendra Kumar Nag [Inde] ; Nidhi Shrivastava ; Jyoti Gupta ; Shailja Misra-BhattacharyaSource :
- Comparative immunology, microbiology and infectious diseases [ 1878-1667 ] ; 2013.
Descripteurs français
- KwdFr :
- Activation des lymphocytes, Activation des macrophages (immunologie), Adhérence cellulaire (immunologie), Animaux, Antigènes d'histocompatibilité de classe II (immunologie), Brugia malayi (immunologie), Clonage moléculaire, Cytokines (immunologie), Cytokines (métabolisme), Cytotoxicité immunologique, Espèces réactives de l'oxygène (métabolisme), Expression des gènes, Facteur d'initiation procaryote 1 (génétique), Facteur d'initiation procaryote 1 (immunologie), Facteur d'initiation procaryote 1 (sécrétion), Femelle, Filariose lymphatique (), Immunoglobuline E (immunologie), Immunoglobuline E (sang), Immunoglobuline G (immunologie), Immunoglobuline G (sang), Immunoglobuline M (immunologie), Immunoglobuline M (sang), Lymphocytes B (immunologie), Lymphocytes auxiliaires Th2 (immunologie), Macrophages (immunologie), Murinae, Mâle, Protéines recombinantes (administration et posologie), Protéines recombinantes (génétique), Protéines recombinantes (immunologie), Réactions croisées (immunologie), Souris, Wolbachia (génétique), Wolbachia (immunologie).
- MESH :
- administration et posologie : Protéines recombinantes.
- génétique : Facteur d'initiation procaryote 1, Protéines recombinantes, Wolbachia.
- immunologie : Activation des macrophages, Adhérence cellulaire, Antigènes d'histocompatibilité de classe II, Brugia malayi, Cytokines, Facteur d'initiation procaryote 1, Immunoglobuline E, Immunoglobuline G, Immunoglobuline M, Lymphocytes B, Lymphocytes auxiliaires Th2, Macrophages, Protéines recombinantes, Réactions croisées, Wolbachia.
- métabolisme : Cytokines, Espèces réactives de l'oxygène.
- sang : Immunoglobuline E, Immunoglobuline G, Immunoglobuline M.
- sécrétion : Facteur d'initiation procaryote 1.
- Activation des lymphocytes, Animaux, Clonage moléculaire, Cytotoxicité immunologique, Expression des gènes, Femelle, Filariose lymphatique, Murinae, Mâle, Souris.
English descriptors
- KwdEn :
- Animals, B-Lymphocytes (immunology), Brugia malayi (immunology), Cell Adhesion (immunology), Cloning, Molecular, Cross Reactions (immunology), Cytokines (immunology), Cytokines (metabolism), Cytotoxicity, Immunologic, Elephantiasis, Filarial (prevention & control), Female, Gene Expression, Histocompatibility Antigens Class II (immunology), Immunoglobulin E (blood), Immunoglobulin E (immunology), Immunoglobulin G (blood), Immunoglobulin G (immunology), Immunoglobulin M (blood), Immunoglobulin M (immunology), Lymphocyte Activation, Macrophage Activation (immunology), Macrophages (immunology), Male, Mice, Murinae, Prokaryotic Initiation Factor-1 (genetics), Prokaryotic Initiation Factor-1 (immunology), Prokaryotic Initiation Factor-1 (secretion), Reactive Oxygen Species (metabolism), Recombinant Proteins (administration & dosage), Recombinant Proteins (genetics), Recombinant Proteins (immunology), Th2 Cells (immunology), Wolbachia (genetics), Wolbachia (immunology).
- MESH :
- chemical , administration & dosage : Recombinant Proteins.
- chemical , blood : Immunoglobulin E, Immunoglobulin G, Immunoglobulin M.
- chemical , genetics : Prokaryotic Initiation Factor-1, Recombinant Proteins.
- chemical , immunology : Cytokines, Histocompatibility Antigens Class II, Immunoglobulin E, Immunoglobulin G, Immunoglobulin M, Prokaryotic Initiation Factor-1, Recombinant Proteins.
- genetics : Wolbachia.
- immunology : B-Lymphocytes, Brugia malayi, Cell Adhesion, Cross Reactions, Macrophage Activation, Macrophages, Th2 Cells, Wolbachia.
- chemical , metabolism : Cytokines, Reactive Oxygen Species.
- prevention & control : Elephantiasis, Filarial.
- chemical , secretion : Prokaryotic Initiation Factor-1.
- Animals, Cloning, Molecular, Cytotoxicity, Immunologic, Female, Gene Expression, Lymphocyte Activation, Male, Mice, Murinae.
Abstract
Wolbachia, the intracellular alpha-proteobacteria are required for the development, fertility and survival of filarial parasites. Wolbachia Translation initiation factor-1 (Wol Tl IF-1) is one of the factors required for Wolbachia growth and viability. In the present study, we cloned, over expressed and purified Wol Tl IF-1 that exhibited strong immuno-reactivity with various categories of bancroftian sera. Immunization with the recombinant protein resulted into significant reduction in microfilarial density (70-72%) and adult worm establishment (61-63%) in susceptible Mastomys coucha. Protection offered by Wol Tl IF-1 was found associated with humoral immune arm as observed by an increased antibody level with preponderance of IgE, IgM, IgG1 and IgG2a isotypes. The anti-Wol Tl IF-1 antibodies promoted profound adherence of peritoneal exudates cells to the surface of microfilariae and infective larvae causing cytotoxicity and their death. The present study indicates potential of recombinant Wol Tl IF-1 as a promising vaccine candidate against human lymphatic filarial infection.
DOI: 10.1016/j.cimid.2012.09.004
PubMed: 23079772
Affiliations:
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(metabolism)</term><term>Recombinant Proteins (administration & dosage)</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (immunology)</term><term>Th2 Cells (immunology)</term><term>Wolbachia (genetics)</term><term>Wolbachia (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Activation des lymphocytes</term><term>Activation des macrophages (immunologie)</term><term>Adhérence cellulaire (immunologie)</term><term>Animaux</term><term>Antigènes d'histocompatibilité de classe II (immunologie)</term><term>Brugia malayi (immunologie)</term><term>Clonage moléculaire</term><term>Cytokines (immunologie)</term><term>Cytokines (métabolisme)</term><term>Cytotoxicité immunologique</term><term>Espèces réactives de l'oxygène (métabolisme)</term><term>Expression des gènes</term><term>Facteur d'initiation procaryote 1 (génétique)</term><term>Facteur d'initiation procaryote 1 (immunologie)</term><term>Facteur d'initiation procaryote 1 (sécrétion)</term><term>Femelle</term><term>Filariose lymphatique ()</term><term>Immunoglobuline E (immunologie)</term><term>Immunoglobuline E (sang)</term><term>Immunoglobuline G (immunologie)</term><term>Immunoglobuline G (sang)</term><term>Immunoglobuline M (immunologie)</term><term>Immunoglobuline M (sang)</term><term>Lymphocytes B (immunologie)</term><term>Lymphocytes auxiliaires Th2 (immunologie)</term><term>Macrophages (immunologie)</term><term>Murinae</term><term>Mâle</term><term>Protéines recombinantes (administration et posologie)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (immunologie)</term><term>Réactions croisées (immunologie)</term><term>Souris</term><term>Wolbachia (génétique)</term><term>Wolbachia (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" 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scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Activation des macrophages</term><term>Adhérence cellulaire</term><term>Antigènes d'histocompatibilité de classe II</term><term>Brugia malayi</term><term>Cytokines</term><term>Facteur d'initiation procaryote 1</term><term>Immunoglobuline E</term><term>Immunoglobuline G</term><term>Immunoglobuline M</term><term>Lymphocytes B</term><term>Lymphocytes auxiliaires Th2</term><term>Macrophages</term><term>Protéines recombinantes</term><term>Réactions croisées</term><term>Wolbachia</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>B-Lymphocytes</term><term>Brugia malayi</term><term>Cell Adhesion</term><term>Cross Reactions</term><term>Macrophage Activation</term><term>Macrophages</term><term>Th2 Cells</term><term>Wolbachia</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cytokines</term><term>Reactive Oxygen Species</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cytokines</term><term>Espèces réactives de l'oxygène</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Immunoglobuline E</term><term>Immunoglobuline G</term><term>Immunoglobuline M</term></keywords><keywords scheme="MESH" type="chemical" qualifier="secretion" xml:lang="en"><term>Prokaryotic Initiation Factor-1</term></keywords><keywords scheme="MESH" qualifier="sécrétion" xml:lang="fr"><term>Facteur d'initiation procaryote 1</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cloning, Molecular</term><term>Cytotoxicity, Immunologic</term><term>Female</term><term>Gene Expression</term><term>Lymphocyte Activation</term><term>Male</term><term>Mice</term><term>Murinae</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Activation des lymphocytes</term><term>Animaux</term><term>Clonage moléculaire</term><term>Cytotoxicité immunologique</term><term>Expression des gènes</term><term>Femelle</term><term>Filariose lymphatique</term><term>Murinae</term><term>Mâle</term><term>Souris</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Wolbachia, the intracellular alpha-proteobacteria are required for the development, fertility and survival of filarial parasites. Wolbachia Translation initiation factor-1 (Wol Tl IF-1) is one of the factors required for Wolbachia growth and viability. In the present study, we cloned, over expressed and purified Wol Tl IF-1 that exhibited strong immuno-reactivity with various categories of bancroftian sera. Immunization with the recombinant protein resulted into significant reduction in microfilarial density (70-72%) and adult worm establishment (61-63%) in susceptible Mastomys coucha. Protection offered by Wol Tl IF-1 was found associated with humoral immune arm as observed by an increased antibody level with preponderance of IgE, IgM, IgG1 and IgG2a isotypes. The anti-Wol Tl IF-1 antibodies promoted profound adherence of peritoneal exudates cells to the surface of microfilariae and infective larvae causing cytotoxicity and their death. The present study indicates potential of recombinant Wol Tl IF-1 as a promising vaccine candidate against human lymphatic filarial infection.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Gupta, Jyoti" sort="Gupta, Jyoti" uniqKey="Gupta J" first="Jyoti" last="Gupta">Jyoti Gupta</name><name sortKey="Misra Bhattacharya, Shailja" sort="Misra Bhattacharya, Shailja" uniqKey="Misra Bhattacharya S" first="Shailja" last="Misra-Bhattacharya">Shailja Misra-Bhattacharya</name><name sortKey="Shrivastava, Nidhi" sort="Shrivastava, Nidhi" uniqKey="Shrivastava N" first="Nidhi" last="Shrivastava">Nidhi Shrivastava</name></noCountry><country name="Inde"><noRegion><name sortKey="Nag, Jeetendra Kumar" sort="Nag, Jeetendra Kumar" uniqKey="Nag J" first="Jeetendra Kumar" last="Nag">Jeetendra Kumar Nag</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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